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kinesin family member 20A OKDB#: 4957
 Symbols: KIF20A Species: human
 Synonyms: MKLP2, RAB6KIFL,MKLP2, RAB6KIFL,  Locus: 5q31 in Homo sapiens


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General Comment
General function Cytoskeleton
Comment
Cellular localization Cytoskeleton
Comment
Ovarian function Oocyte maturation, Early embryo development , First polar body extrusion
Comment KIF20A Regulates Porcine Oocyte Maturation and Early Embryo Development. Zhang Y 2014 et al. KIF20A (Kinesin-like family member 20A), also called mitotic kinesin-like proteins 2 (MKLP2), is a mammalian mitotic kinesin-like motor protein of the Kinesin superfamily proteins (KIFs), which was originally involved in Golgi apparatus dynamics and thought to essential for cell cycle regulation during successful cytokinesis. In the present study, we investigated whether KIF20A has roles on porcine oocyte meiotic maturation and subsequent early embryo development. By immunofluorescence staining, KIF20A was found to exhibit a dynamic localization pattern during meiosis. KIF20A was restricted to centromeres after germinal vesicle breakdown (GVBD), transferred to the midbody at telophase I (TI), and again associated with centromeres at metaphase II (MII). Inhibition of endogenous KIF20A via a specific inhibitor, Paprotrain, resulted in failure of polar body extrusion. Further cell cycle analysis showed that the percentage of oocytes that arrested at early metaphase I (MI) stage increased after KIF20A activity inhibition; however, the proportion of oocytes at anaphase/telophase I (ATI) and MII stages decreased significantly. Our results also showed that KIF20A inhibition did not affect spindle morphology. In addition, KIF20A was localized at the nucleus of early embryos, and KIF20A inhibition resulted in failure of early parthenogenetic embryo development. These results demonstrated that KIF20A is critical for porcine oocyte meiotic maturation and subsequent early embryo development. /////////////////////////
Expression regulated by
Comment
Ovarian localization Oocyte
Comment MKlp2 inhibitior paprotrain affects polar body extrusion during mouse oocyte maturation. Liu J 2013 et al. BACKGROUND Mammalian oocyte meiotic maturation involves a number of important processes, including spindle assembly and migration, cortical reorganization and polar body extrusion. Numerous proteins contribute to these processes, but it is unknown whether MKlp2 (mitotic kinesin-like protein 2; also called KIF20A), a microtubule-associated protein that regulates cytokinesis during mitosis, is involved in oocyte maturation. METHODS Confocal microscopy, time lapse microscopy, inhibitor treatment were adopted to examine the roles of MKlp2 in mouse oocyte. RESULTS Immunostaining results showed that MKlp2 localized to oocyte microtubules. Time-lapse microscopy showed that disrupting MKlp2 expression with paprotrain, a specific inhibitor of MKlp2, resulted in polar body extrusion failure. This could be rescued after rescuing oocytes from paprotrain in fresh medium. Cell cycle analysis showed that most oocytes were arrested at metaphase I or telophase I. However, oocyte spindle structure and chromosome alignment were not disrupted after the inhibition of MKlp2 by paprotrain. CONCLUSIONS This study demonstrated that MKlp2 is crucial for oocyte maturation by regulating polar body extrusion. /////////////////////////
Follicle stages
Comment
Phenotypes
Mutations 0 mutations
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created: Dec. 24, 2013, 4:47 p.m. by: hsueh   email:
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last update: Aug. 5, 2014, 10:42 a.m. by: hsueh    email:



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