| SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) | OKDB#: 4414 |
| Symbols: | SPC25 | Species: | human | ||
| Synonyms: | AD024, SPBC25, hSpc25, MGC22228, | Locus: | 2q31.1 in Homo sapiens |
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| General Comment | NCBI Summary: This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq] | ||||
| General function | Chromosome organization | ||||
| Comment | |||||
| Cellular localization | Nuclear | ||||
| Comment | |||||
| Ovarian function | Oocyte maturation | ||||
| Comment | Spc25?-?How the kinetochore protein plays during oocyte meiosis. Xiong B et al. Comment on: Sun SC, et al. Cell Cycle 2010; 9:4552-9. | ||||
| Expression regulated by | |||||
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| Ovarian localization | Oocyte | ||||
| Comment | Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes. Sun SC et al. Spc25 is a component of the Ndc80 complex which consists of Ndc80, Nuf2, Spc24 and Spc25. Previous work has shown that Spc25 is involved in regulation of kinetochore microtubule attachment and the spindle assembly checkpoint in mitosis. the roles of Spc25 in meiosis remain unknown. Here, we report its expression, localization and functions in mouse oocyte meiosis. The Spc25 mRNA level gradually increased from the GV to MI stage, but decreased by MII during mouse oocyte meiotic maturation. Immunofluorescent staining showed that Spc25 was restricted to the germinal vesicle, and associated with chromosomes during all stages after GVBD. Overexpression of Spc25 by mRNA injection resulted in oocyte meiotic arrest, chromosome misalignment and spindle disruption. Conversely, Spc25 RNAi by siRNA injection resulted in precocious polar body extrusion and caused severe chromosome misalignment and aberrant spindle formation. Our data suggest that Spc25 is required for chromosome alignment, spindle formation and proper spindle checkpoint signaling during meiosis. | ||||
| Follicle stages | Preovulatory | ||||
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| Phenotypes | |||||
| Mutations | 0 mutations | ||||
| Genomic Region | show genomic region | ||||
| Phenotypes and GWAS | show phenotypes and GWAS | ||||
| Links |
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| created: | Nov. 24, 2010, 6:31 a.m. | by: |
hsueh email:
home page: |
| last update: | March 23, 2011, 9:19 a.m. | by: | hsueh email: |
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