NCBI Summary:
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]
General function
Enzyme
Comment
Cellular localization
Secreted
Comment
Ovarian function
Follicle endowment, Ovulation, Follicle rupture
Comment
Expression regulated by
LH
Comment
Gene expression profiling of bovine preovulatory follicles: Gonadotropin surge and prostanoid dependent up-regulation of genes potentially linked to the ovulatory process. Li Q et al. The molecular mechanisms of ovulation and luteinization have not been well established, partially due to lack of a comprehensive understanding of functionally significant genes up-regulated in response to an ovulatory stimulus and the signaling pathways involved. In the present study, transcripts increased in bovine preovulatory follicles following a GnRH-induced LH surge were identified using microarray technology. Increased expression of 368 and 878 genes was detected at 12 (368 genes) and 20 h (878 genes) following GnRH injection. The temporal, cell specific and prostanoid dependent regulation of selected genes (ADAM10, DBI, CD36, MTSS1, TFG, and RABGAP1) identified from microarray studies and related genes (ADAM17, AREG) of potential significance were also investigated. Expression of mRNA for DBI and CD36 was simultaneously up-regulated in theca and granulosa cells following the LH surge, whereas temporal regulation of ADAM10, MTSS1, TFG and RABGAP1 was distinct in the two cell compartments and increased granulosa TFG and RABGAP1 mRNA was prostanoid dependent. AREG mRNA was increased in theca and granulosa cells at 12 and 24 h following GnRH injection. ADAM17 mRNA was increased in theca, but reduced in granulosa cells 24 h following GnRH injection. The increased ADAM17 and AREG mRNA was prostanoid dependent. ADAM10 and ADAM17 protein were increased specifically in the apex but not the base of preovulatory follicles and the increase in ADAM17 was prostanoid dependent. Results reveal novel information on regulation of preovulatory gene expression and suggest a potential functional role for ADAM10 and ADAM17 proteins in the region of follicle rupture.
Ovarian localization
Comment
Follicle stages
Comment
Phenotypes
Mutations
1 mutations
Species: mouse
Mutation name: type: null mutation fertility: subfertile Comment: ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. Feng L et al. (2016) Ovarian follicles are the basic functional units of female reproduction in the mammalian ovary. We show here that A Disintegrin and Metalloproteinase Domain 10 (ADAM10), a cell surface sheddase, plays an indispensable role in controlling primordial follicle formation by regulating the recruitment of follicle supporting cells in mice. We demonstrate that suppressing ADAM10in vitroor deletionAdam10 in vivodisrupts germline cyst breakdown and primordial follicle formation. Using a cell lineage tracing approach, we show that ADAM10 governs the recruitment of ovarian follicle cells by regulating the differentiation and proliferation of LGR5(+)follicle supporting progenitor cells. By detecting the development of FOXL2(+)pregranulosa cells, we found that inhibiting ADAM10 reduced the number of FOXL2(+)cells in perinatal ovaries. Furthermore, inhibiting ADAM10 suppressed the activation of Notch signaling, and blocking Notch signaling also disrupted the recruitment of follicle progenitor cells. We found that ADAM10-Notch signaling in ovarian somatic cells governs the primordial follicle formation by controlling the development of ovarian pregranulosa cells. The proper recruitment of ovarian follicle supporting cells is essential for the establishment of ovarian reserve in mice.//////////////////