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General Comment |
Yoon SJ, et al reported the identification of differential gene expression in germinal vesicle vs. metaphase II mouse oocytes by using annealing control primers.
By using a new innovative technology, annealing control primer-polymerase chain reaction (ACP-PCR), we identified genes that are differentially expressed in immature GV vs. in mature MII mouse oocytes. The results of the present study will be valuable in understanding the components of oocyte maturation and provide a basis for studies of human oocyte maturation.
Interestingly, we identified two genes previously reported as testis-specific genes, Fsrg3 and TEMO, as oocyte-expressed genes (20, 21 and 22). Fsrg3, also known as BRDT, is involved in chromatin remodeling, and TEMO is a marker for Sertoli cellgerm cell interactions, but its specific function is unknown. Characterization of the expression and function of these genes in oocyte maturation should be addressed.
In conclusion, this is the first report identifying genes that are differentially expressed in GV and MII oocytes using the new ACP technology. The results of the present study with the mouse model will be valuable in understanding the mechanisms of oocyte maturation and will provide a basis for studies of human oocyte maturation.
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