Stanford Home
Ovarian Kaleidoscope Database (OKdb)

Home

History

Transgenic Mouse Models

INFORGRAPHICS

Search
Submit
Update
Chroms
Browse
Admin

Hsueh lab

HPMR

Visits
since 01/2001:
176557

Chemokine, Cc Motif, Ligand 25 OKDB#: 2818
 Symbols: CCL25 Species: human
 Synonyms: SMALL INDUCIBLE CYTOKINE SUBFAMILY A, MEMBER 25, FORMERLY, SCYA25, FORMERLY|THYMUS-EXPRESSED CHEMOKINE, TECK  Locus: 19p13.2 in Homo sapiens


For retrieval of Nucleotide and Amino Acid sequences please go to: OMIM   UCSC Genome Browser   GEO Profiles new!   Amazonia (transcriptome data) new!
R-L INTERACTIONS   MGI

DNA Microarrays
SHOW DATA ...
link to BioGPS
General Comment
General function Ligand, Hormone, Cytokine
Comment
Cellular localization Secreted
Comment
Ovarian function Ovulation
Comment Transient Expression of CC Chemokine TECK in the Ovary during Ovulation: Its Potential Role in Ovulation Zhou C, et al . Chemokine thymus-expressed chemokine (TECK), which is expressed exclusively in the thymus and small intestine, plays a critical role in T-cell development. Our previous study revealed its expression in the ovary also. This study investigated its ovarian expression during ovulatory process. Method of study: Super-ovulation was induced in young female CD1 mice by equine chorionic gonadotropin (eCG) and human chorionic gonadotropic (hCG). Ovarian TECK expression during ovulation was determined by: (1) reverse transcriptase-polymerase chain reaction (RT-PCR) at mRNA level, (2) Western blot and immunohistology at the protein level, and (3) leukocyte infiltration assay at the bioactive level. Results: A transient, high-level expression of TECK in murine ovaries at the mRNA level during hCG-induced ovulation was detected. Sequencing of directly cloned PCR product confirmed the ovarian expression of TECK. The peak expression of TECK was observed at 10-12 hr post-hCG injection; real-time PCR revealed an 800-fold increase during its expression peak over 0 hr. The expressed ovarian TECK protein was readily detectable by Western blot. Immunohistochemistry localized TECK expression to the ovarian interstitial tissue surrounding, or in the theca layer of the mature follicles undergoing ovulatory process. Expression of TECK receptor, the CC chemokine receptor (CCR9) was also detected in the ovulating ovaries. Using in vitro leukocyte infiltration assay, we first demonstrated that ovaries undergoing the ovulatory process were able to selectively chemoattract mononuclear cells. Importantly, neutralization of TECK by the antibody resulted in a 85% reduction in the chemotactic activities of the ovaries. Conclusion: This study suggested that ovarian expression of TECK is under a tight hormonal regulation, and expressed TECK may be responsible for recruitment of mononuclear cells into the ovary to participate in the ovulatory process. Potential roles of a special CD8alpha alpha+ cell population and CC chemokine thymus-expressed chemokine in ovulation related inflammation. Zhou C et al. It is well known that ovulation may be an inflammatory process. However, it remains elusive how immune cells participate in this process. We have identified a novel CD8alpha alpha(+) population, which resembles tissue dendritic cells, in the theca of antral follicles. We further observed a dramatic influx of the CD8alpha alpha(+) cells into the ovulating follicles. This CD8alpha alpha(+) population was absent in the ovary of estradiol-induced anovulatory C31F(1) mice and subfertile athymic nude mice. Expression of a CC chemokine thymus-expressed chemokine (TECK) has previously been found in the ovary; we further demonstrated that TECK attracted CD8alpha alpha(+) cells into the ovary. Anti-TECK Ab, elicited in the female mice by active immunization, depleted the ovarian CD8alpha alpha(+) cells in vivo. Mice with a high titer of TECK Ab failed to ovulate after superovulation induction. More importantly, the immunized mice had greatly reduced fertility, which was positively correlated with the Ab titers. Ovarian TECK expression was normal in anovulatory C31F(1) mice, suggesting that infertility in the immunized mice is due to a block of CD8alpha alpha(+) cell migration. Finally, the origin of ovarian CD8alpha alpha(+) cells was explored. Upon being transferred, thymic CD8alpha(+) cells were able to home to the theca of follicles in the recipients. Thus, ovarian CD8alpha alpha(+) cells, which participate in the ovulation-related inflammation, may originate in the thymus.
Expression regulated by LH
Comment
Ovarian localization
Comment
Follicle stages
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
OMIM \ Animal Model
KEGG Pathways
Recent Publications
None
Search for Antibody


created: April 20, 2005, 2:10 p.m. by: hsueh   email:
home page:
last update: Jan. 3, 2009, 1:32 p.m. by: hsueh    email:



Use the back button of your browser to return to the Gene List.

Click here to return to gene search form