| ADAM metallopeptidase with thrombospondin type 1 motif 9 | OKDB#: 1421 |
| Symbols: | ADAMTS9 | Species: | human | ||
| Synonyms: | Locus: | 3p14.1 in Homo sapiens |
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| General Comment |
ADAMTS proteins are structurally homologous to ADAM proteins, but they also contain at least 1 C-terminal
thrombospondin type 1 (TSP1) repeat and are secreted rather than membrane bound. Members of the ADAM-TS
family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the
inhibition of angiogenesis.
NCBI Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016] |
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| General function | |||||
| Comment | |||||
| Cellular localization | Secreted | ||||
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| Ovarian function | |||||
| Comment | Induction of proteinases in the human preovulatory follicle of the menstrual cycle by human chorionic gonadotropin. Rosewell KL et al. (2014) To explore the temporal expression in granulosa and theca cells of key members of the MMP and ADAMTS families across the periovulatory period in women to gain insight into their possible roles during ovulation and early luteinization. Experimental prospective clinical study and laboratory-based investigation. University medical center and private IVF center. Thirty-eight premenopausal women undergoing surgery for tubal ligation and six premenopausal women undergoing assisted reproductive techniques. Administration of hCG and harvesting of follicles by laparoscopy and collection of granulosa-lutein cells at oocyte retrieval. Expression of mRNA for matrix metalloproteinase (MMPs) and the A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS) in human granulosa cells and theca cells collected across the periovulatory period of the menstrual cycle and in cultured granulosa-lutein cells after hCG. Localization of MMPs and ADAMTSs by immunohistochemistry. Expression of MMP1 and MMP19 mRNA increased in both granulosa and theca cells after hCG administration. ADAMTS1 and ADAMTS9 mRNA increased in granulosa cells after hCG treatment, however, thecal cell expression for ADAMTS1 was unchanged, while ADAMTS9 expression was decreased. Expression of MMP8 and MMP13 mRNA was unchanged. Immunohistochemistry confirmed the localization of MMP1, MMP19, ADAMTS1, and ADAMTS9 to the granulosa and thecal cell layers. The collection of the dominant follicle throughout the periovulatory period has allowed the identification of proteolytic remodeling enzymes in the granulosa and theca compartments that may be critically involved in human ovulation. These proteinases may work in concert to regulate breakdown of the follicular wall and release of the oocyte.////////////////// | ||||
| Expression regulated by | LH | ||||
| Comment | Gene expression profiling of upregulated mRNAs in granulosa cells of bovine ovulatory follicles following stimulation with hCG. Lussier JG et al. (2017) Ovulation and luteinization of follicles are complex biological processes initiated by the preovulatory luteinizing hormone surge. The objective of this study was to identify genes that are differentially expressed in bovine granulosa cells (GC) of ovulatory follicles. Granulosa cells were collected during the first follicular wave of the bovine estrous cycle from dominant follicles (DF) and from ovulatory follicles (OF) obtained 24 h following injection of human chorionic gonadotropin (hCG). A granulosa cell subtracted cDNA library (OF-DF) was generated using suppression subtractive hybridization and screened. Detection of genes known to be upregulated in bovine GC during ovulation, such as ADAMTS1, CAV1, EGR1, MMP1, PLAT, PLA2G4A, PTGES, PTGS2, RGS2, TIMP1, TNFAIP6 and VNN2 validated the physiological model and analytical techniques used. For a subset of genes that were identified for the first time, gene expression profiles were further compared by semiquantitative RT-PCR in follicles obtained at different developmental stages. Results confirmed an induction or upregulation of the respective mRNAs in GC of OF 24 h after hCG-injection compared with those of DF for the following genes: ADAMTS9, ARAF, CAPN2, CRISPLD2, FKBP5, GFPT2, KIT, KITLG, L3MBLT3, MRO, NUDT10, NUDT11, P4HA3, POSTN, PSAP, RBP1, SAT1, SDC4, TIMP2, TNC and USP53. In bovine GC, CRISPLD2 and POSTN mRNA were found as full-length transcript whereas L3MBLT3 mRNA was alternatively spliced resulting in a truncated protein missing the carboxy-terminal end amino acids, (774)KNSHNEL(780). Conversely, L3MBLT3 is expressed as a full-length mRNA in a bovine endometrial cell line. The (774)KNSHNEL(780) sequence is well conserved in all mammalian species and follows a SAM domain known to confer protein/protein interactions, which suggest a key function for these amino acids in the epigenetic control of gene expression. We conclude that we have identified novel genes that are upregulated by hCG in bovine GC of OF, thereby providing novel insight into peri-ovulatory regulation of genes that contribute to ovulation and/or luteinization processes.////////////////// | ||||
| Ovarian localization | Granulosa, Theca | ||||
| Comment | HIGHLY EXPRESSED IN ALL FETAL TISSUES. EXPRESSED SLIGHTLY IN ADULT OVARY, PANCREAS, HEART, KIDNEY, LUNG, PLACENTA. ALSO DETECTED IN SPINAL CORD AND BRAIN. NOT DETECTED IN COLON, SMALL INTESTINE, TESTIS, LIVER, SKELETAL MUSCLE, SPLEEN OR THYMUS. | ||||
| Follicle stages | |||||
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| Phenotypes | |||||
| Mutations |
4 mutations
Species: C. elegans
Species: D. melanogaster
Species: None
Species: mouse
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| Genomic Region | show genomic region | ||||
| Phenotypes and GWAS | show phenotypes and GWAS | ||||
| Links |
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| created: | Jan. 19, 2002, 10:01 a.m. | by: |
hsueh email:
home page: |
| last update: | May 6, 2021, 1:14 p.m. | by: | hsueh email: |
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