Proteinase-3 is one of the antibiotic proteins of neutrophilic granules belonging to the serine protease family. It is
closely related to 2 others: neutrophil elastase (ELA2; OMIM 130130) and azurocidin (AZU; OMIM 162815).
General function
Enzyme, Peptidase/Protease
Comment
Cellular localization
Secreted
Comment
Ovarian function
Comment
Expression regulated by
Comment
Ovarian localization
Comment
Follicle stages
Antral, Preovulatory
Comment
Liu HC, et al 2001 reported tha application of complementary DNA microarray (DNA chip) technology in the study of gene expression profiles during
folliculogenesis.
They used oligonucleotide microarray (DNA chip)-based hybridization
analysis to gain a comprehensive view of gene expression and regulation
involved in folliculogenesis.
Preantral follicles isolated from day 14 B6D2F-1 mice
were stimulated in vitro to form Graafian follicles. Total RNA extracted from
the mouse preantral and Graafian follicles were reverse transcribed, labeled
with digoxigenin-11-dUTP, and then hybridized with Clontech Atlas mouse cDNA
expression arrays for comparison. Of 588 known studied genes, 39 and 61 were detected in preantral follicles and in Graafian follicles, respectively, and 17 were highly
expressed consistently in both preantral and Graafian follicles. Performing
clustering analysis, 15 detected genes were down-regulated and
46 were upregulated as the follicles advanced to mature stages. The Proteinase gene is down-regulated in the Graafian follicles.