Takayama et
al. (1995) used interaction cloning to identify a mouse protein that binds to Bcl2. The protein, referred to as
Bcl2-associated athanogene-1 (BAG1), is rich in glutamic acid residues. Overexpression of recombinant Bag1 in 3T3
fibroblasts prevented them from undergoing apoptosis when deprived of serum.
NCBI Summary:
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of alternative translation initiation sites, including a non-AUG site.
General function
Cell death/survival, Apoptosis
Comment
Cellular localization
Comment
Ovarian function
Follicle atresia
Comment
Expression regulated by
Comment
Ovarian localization
Comment
Follicle stages
Antral, Preovulatory
Comment
Liu HC, et al 2001 reported tha application of complementary DNA microarray (DNA chip) technology in the study of gene expression profiles during
folliculogenesis.
They used oligonucleotide microarray (DNA chip)-based hybridization
analysis to gain a comprehensive view of gene expression and regulation
involved in folliculogenesis.
Preantral follicles isolated from day 14 B6D2F-1 mice
were stimulated in vitro to form Graafian follicles. Total RNA extracted from
the mouse preantral and Graafian follicles were reverse transcribed, labeled
with digoxigenin-11-dUTP, and then hybridized with Clontech Atlas mouse cDNA
expression arrays for comparison. Of 588 known studied genes, 39 and 61 were detected in preantral follicles and in Graafian follicles, respectively, and 17 were highly
expressed consistently in both preantral and Graafian follicles.
,including Bcl2-associated Athanogene 1.