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Protein kinase C lamda OKDB#: 1259
 Symbols: PKC lambda Species: human
 Synonyms:  Locus:


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General Comment Members of the protein kinase C (PKC) family of serine/threonine kinases play critical roles in the regulation of cellular differentiation and proliferation of diverse cell types. Protein kinase C (PKC) is the major phorbol ester receptor. Nine mammalian members of the PKC family have been identified and designated alpha, beta, gamma, delta, epsilon, zeta, eta, theta, and lambda.

General function
Comment
Cellular localization Cytoplasmic
Comment
Ovarian function Steroid metabolism
Comment
Expression regulated by Growth Factors/ cytokines
Comment Peluso JJ, et al reported that basic fibroblast growth factor maintains calcium homeostasis and granulosa cell viability by stimulating calcium efflux via a PKC delta-dependent pathway. Previous studies have demonstrated that basic fibroblast growth factor prevents granulosa cell apoptosis. Granulosa cells, as well as spontaneously immortalized granulosa cells, were shown to express PKC delta, -lambda, and -zeta. Finally, the PKC delta -specific inhibitor, rottlerin, blocked basic fibroblast growth factor's antiapoptotic action in granulosa cells and spontaneously immortalized granulosa cells. These studies suggest that basic fibroblast growth factor regulates intracellular free calcium through a PKC delta -dependent mechanism and that a sustained increase in intracellular free calcium is sufficient to induce and is required for granulosa cell apoptosis.
Ovarian localization Granulosa, Luteal cells
Comment Tai CJ, et al 2001 reported the antigonadotropic action of adenosine triphosphate in human granulosa-luteal cells and the involvement of protein kinase c alpha. The presence of P2U purinoceptor in human granulosa-luteal cells (hGLCs) indicates a potential role of ATP in regulating ovarian function and an inhibitory effect of ATP on hCG-induced cAMP production was observed. Extracellular ATP has been shown to activate protein kinase C (PKC) after binding to a purinoceptor. To further investigate the involvement of PKC isoforms in mediating the inhibitory effect of ATP, the presence of PKC isoforms in cultured human granulosa luteal cells (hGLCs) was examined by Western blot using monoclonal antibodies against specific isoforms. Translocation of PKC isoforms from cytosolic fraction to membrane fraction was studied to identify the active PKC isozymes subsequent to ATP treatment. The change in PKC isoform in PKC-depleted cells was also examined. The results demonstrated the presence of PKC alpha, -delta, -iota, and -lambda isoforms in hGLCs and the translocation of PKCa subsequent to ATP treatment. In PKC-depleted cells the PKCa level was reduced, and no significant effect of ATP on hCG-stimulated cAMP production was observed.
Follicle stages Preovulatory, Corpus luteum
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
Recent Publications
http://stke.sciencemag.org/cgi/cm/CMP_5533
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created: Aug. 8, 2001, 3:23 p.m. by: hsueh   email:
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last update: Oct. 20, 2001, 2:36 a.m. by: system    email:



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