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HPMR

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Protein kinase C-iota OKDB#: 1258
 Symbols: PKC-iota Species: human
 Synonyms:  Locus:


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General Comment Members of the protein kinase C (PKC) family of serine/threonine kinases play critical roles in the regulation of cellular differentiation and proliferation of diverse cell types. Protein kinase C (PKC) is the major phorbol ester receptor. Nine mammalian members of the PKC family have been identified and designated alpha, beta, gamma, delta, epsilon, zeta, eta, theta, and lambda.

General function
Comment
Cellular localization Cytoplasmic
Comment
Ovarian function Steroid metabolism
Comment
Expression regulated by
Comment
Ovarian localization Granulosa, Luteal cells
Comment Tai CJ, et al 2001 reported the antigonadotropic action of adenosine triphosphate in human granulosa-luteal cells and the involvement of protein kinase c alpha. The presence of P2U purinoceptor in human granulosa-luteal cells (hGLCs) indicates a potential role of ATP in regulating ovarian function and an inhibitory effect of ATP on hCG-induced cAMP production was observed. Extracellular ATP has been shown to activate protein kinase C (PKC) after binding to a purinoceptor. To further investigate the involvement of PKC isoforms in mediating the inhibitory effect of ATP, the presence of PKC isoforms in cultured human granulosa luteal cells (hGLCs) was examined by Western blot using monoclonal antibodies against specific isoforms. Translocation of PKC isoforms from cytosolic fraction to membrane fraction was studied to identify the active PKC isozymes subsequent to ATP treatment. The change in PKC isoform in PKC-depleted cells was also examined. The results demonstrated the presence of PKC alpha, -delta, -iota, and -lambda isoforms in hGLCs and the translocation of PKCa subsequent to ATP treatment. In PKC-depleted cells the PKCa level was reduced, and no significant effect of ATP on hCG-stimulated cAMP production was observed.
Follicle stages Preovulatory, Corpus luteum
Comment
Phenotypes
Mutations 0 mutations
Genomic Region show genomic region
Phenotypes and GWAS show phenotypes and GWAS
Links
Recent Publications
http://stke.sciencemag.org/cgi/cm/CMP_5533
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created: Aug. 8, 2001, 3:18 p.m. by: hsueh   email:
home page:
last update: Aug. 29, 2001, 10:15 a.m. by: system    email:



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